Surface Visualisation and 3D Feature Analysis in Confocal Microscopy Images

Chondrons form the fundamental biomechanical and metabolic unit of articular cartilage. But there is no accurate description of chondron volume which is known to change when articular cartilage is functionally loaded. This report discusses the 3D feature analysis of the confocal microscopy images of chondrons. The basic principle of confocal microscopy is described. The segmentation problem of confocal images is also discussed. Two different reconstruction algorithms, Marching Cubes and Dividing Cubes are also illustrated and their respectively ways of calculating volume and surface area are also discussed. The results of calculating 3D features of sperical polysterene beads using Marching Cubes algorithms are also given. 1 The University of Auckland, Computer Science Department, CITR, Tamaki Campus (Building 731), Glen Innes, Auckland, New Zealand 2 Department of Anatomy, Faculty of Medicine and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand Surface Visualisation and 3D Feature Analysis in Confocal Microscopy Images Fu Cheong Fong , Reinhard Klette , Anthony Poole , and Man Hon Wong 1 1 CITR, University of Auckland Tamaki Campus, Building 731, Auckland, New Zealand 2 Department of Anatomy Faculty of Medicine and Health Science, University of Auckland Private Bag 92019, Auckland, New Zealand Abstract: Chondrons form the fundamental biomechanical and metabolic unit of articular cartilage. But there is no accurate description of chondron volume which is known to change when articular cartilage is functionally loaded. This report discusses the 3D feature analysis of the confocal microscopy images of chondrons. The basic principle of confocal microscopy is described. The segmentation problem of confocal images is also discussed. Two di erent reconstruction algorithms, Marching Cubes and Dividing Cubes are also illustrated and their respectively ways of calculating volume and surface area are also discussed. The results of calculating 3D features of spherical polystyrene beads using Marching Cubes algorithm are also given. Chondrons form the fundamental biomechanical and metabolic unit of articular cartilage. But there is no accurate description of chondron volume which is known to change when articular cartilage is functionally loaded. This report discusses the 3D feature analysis of the confocal microscopy images of chondrons. The basic principle of confocal microscopy is described. The segmentation problem of confocal images is also discussed. Two di erent reconstruction algorithms, Marching Cubes and Dividing Cubes are also illustrated and their respectively ways of calculating volume and surface area are also discussed. The results of calculating 3D features of spherical polystyrene beads using Marching Cubes algorithm are also given.